anti-hsp60/hspd1 antibody cat#ba1511  (Boster Bio)

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: Cell migration capability of EA.hy926 cells after treatment with shHSPD1-A549 and shControl-A549 secretomes. (A) Representative images of the wound area captured at 0, 8, 16 and 24 h after secretome treatment, captured at x100 magnification. (B) Percentage of wound healing. Data are presented as mean ± SD from three independent experiments. * P<0.05. sh, short hairpin; HSPD1, heat shock protein family D member 1.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques: Migration

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: Cell invasion of EA.hy926 cells after treatment with shHSPD1-A549 and shControl-A549 secretomes. (A) Representative images of crystal violet-stained EA.hy926 cells after 24-h treatment with secretomes, captured at x100 magnification. (B) The optical density of crystal violet-stained cells at 590 nm. Data are presented as mean ± SD from three independent experiments and expressed as a fold change relative to the untreated control. *** P<0.001. sh, short hairpin; HSPD1, heat shock protein family D member 1.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques: Staining, Control

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: Cell aggregation of EA.hy926 cells following treatment with shHSPD1-A549 and shControl-A549 secretomes. (A) Representative images of EA.hy926 cell aggregates after secretome treatment, captured at x100 magnification. (B) Size of cell aggregates. Data are presented as mean ± SD from three independent experiments. * P<0.05, ** P<0.01. sh, short hairpin; HSPD1, heat shock protein family D member 1.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques:

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: Endothelial tube formation of EA.hy926 cells after treatment with shHSPD1-A549 and shControl-A549 secretomes. (A) Representative images of tube formation in EA.hy926 cells after secretome treatment, captured at x40 magnification with additional zoomed-in areas. (B) Length of formed tubes. (C) Area of formed tubes. Data are presented as mean ± SD from three independent experiments. * P<0.05. sh, short hairpin; HSPD1, heat shock protein family D member 1.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques:

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: VEGF levels in the culture supernatant of EA.hy926 cells after treatment with shHSPD1-A549 and shControl-A549 secretomes. VEGF levels were measured by ELISA. The bar graph represents mean ± SD from three independent experiments. * P<0.05, ** P<0.01. VEGF, vascular endothelial growth factor; sh, short hairpin; HSPD1, heat shock protein family D member 1.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Biomedical Reports

Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells

doi: 10.3892/br.2025.1955

Figure Lengend Snippet: Correlation of VEGFA expression with HSPD1 expression and overall survival in patients with lung adenocarcinoma. (A) Scatter plot showing the correlation between VEGFA and HSPD1 expression in the TCGA-LUAD cohort, analyzed using the TIMER database. (B) Kaplan-Meier survival curve comparing overall survival in lung adenocarcinoma patients with low and high VEGFA expression, obtained from the KM Plotter database. VEGF, vascular endothelial growth factor; sh, short hairpin; HSPD1, heat shock protein family D member 1; TCGA-LUAD, The Cancer Genome Atlas-Lung Adenocarcinoma; TIMER, Tumor IMmune Estimation Resource.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in phosphate-buffered saline (PBS) at room temperature for 1 h. Subsequently, the membrane was incubated overnight at 4˚C with either rabbit monoclonal anti-HSPD1 (1:1,000; cat. no. 12165; Cell Signaling Technology, Inc.) or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam).

Techniques: Expressing